Analar. Prepare arsenomolybdate reagent in three steps: Dissolve 25 g ammonium molybdate in 400 mL water and add 25 mL concentrated sulfuric acid and mix. Dilute to a final volume of 100cm 3 with water. Pipette out standard sugar solution in the range of 0 to 3 mL in different test tubes and make up the volume of all test tubes to 3 mL with distilled water concentrations ranging from 0 to 750 mg. Add 1 mL DNS reagent to all the test tubes and mix plug the test tube with cotton or marble and keep the test tube in a boiling water bath for 5 minute. After cooling to room temperature in a cold water bath, record the absorbance with a spectrophotometer at 540nm. This article is cited by 15145 publications. 12 PrepMan Ultra Sample Preparation Reagent Protocol About the PrepMan Ultra Sample Preparation Reagent Purpose of PrepMan Ultra Sample Preparation Reagent PrepMan® Ultra Sample Preparation Reagent provides a simple way to prepare DNA from a wide range of sample types including: † Processed foods and their ingredients † Bacteria † Fungi Use a good quality starch, e.g. Mix and heat gently to make a uniform suspension. DNS method The DNS method for estimating the concentration of reducing sugars in a sample Reducing sugars contain free carbonyl group, have the property to reduce many of the reagents. 7.4.4 Cellulase Enzyme Solution (Cellulase) 7.4.4.1 Immediately before use, prepare a solution containing 2-6 units/ml of Cellulase in cold deionized water. G3293. Dissolve contents per label instructions. The colour of the reagent changes from yellow to orange or red, depending upon the concentration of reducing sugar present. All monosaccaride and some disaccaride are reducing sugars v v … No. Keep in boiling water bath for 15 minutes. necessary. Thamara C. Coutinho, João O. D. Malafatti, Elaine C. Paris, Paulo W. Tardioli, Cristiane S. Farinas. 4H 2 O) Add 20cm 3 of 2N NaOH. Add 3 ml of DNS reagent to 3 ml of glucose sample in a lightly capped test tube. Take 7 clean, dry test tubes. Weigh out 2 g starch powder. This starch solution does not keep well and should be made up fresh on the required day. Make dilutions of glucose standards; Add 3 ml of DNSA reagent to all the eight test tubes. Dissolve 3 g sodium arsenate heptahydrate in 25 mL water. The DNSA reagent base is supplied without sodium hydroxide. Mix well. This stock solution is stable for at least 2 weeks at room temperature. 7.4.3.1 Use Glucose (HK) Assay Reagent, Prod. Generate a calibration curve to correlate the absorbance to the sucrose concentration. 7.4.3 Glucose (HK) Determination Vial. 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